goat anti human il10 antibodies Search Results


biotinylated detection antibody  (R&D Systems)
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R&D Systems biotinylated detection antibody
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th 17 cytokines  (R&D Systems)
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R&D Systems th 17 cytokines
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goat anti human il10 antibodies  (R&D Systems)
88
R&D Systems goat anti human il10 antibodies
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Goat Anti Human Il10 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal goat anti mouse il 10 ab  (R&D Systems)
93
R&D Systems monoclonal goat anti mouse il 10 ab
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Monoclonal Goat Anti Mouse Il 10 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human il 10 antibody  (R&D Systems)
92
R&D Systems goat anti human il 10 antibody
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Goat Anti Human Il 10 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti il10 antibodies  (R&D Systems)
94
R&D Systems anti il10 antibodies
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Anti Il10 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal goat igg anti human il 10 blocking antibody  (R&D Systems)
97
R&D Systems polyclonal goat igg anti human il 10 blocking antibody
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Polyclonal Goat Igg Anti Human Il 10 Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse il 22  (R&D Systems)
93
R&D Systems goat anti mouse il 22
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Goat Anti Mouse Il 22, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 10  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology il 10
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Il 10, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human il 22 antibody ab  (R&D Systems)
93
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FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
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goat anti human ifn receptor il 10r  (R&D Systems)
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R&D Systems goat anti human ifn receptor il 10r
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
Goat Anti Human Ifn Receptor Il 10r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated goat anti mouse il 22 antibody  (R&D Systems)
96
R&D Systems biotinylated goat anti mouse il 22 antibody
FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).
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Image Search Results


FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

Journal: Human gene therapy

Article Title: Helper-dependent adenoviral vector achieves prolonged, stable expression of interleukin-10 in rabbit carotid arteries but does not limit early atherogenesis.

doi: 10.1089/hum.2010.175

Figure Lengend Snippet: FIG. 1. Helper-dependent adenoviral (HDAd) vectors ex- press interleukin (IL)-10 protein in vitro. (a) Expression cas- sette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, b-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 50-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 30-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAd- gIL-10, at the viral doses indicated (particles/ml). Condi- tioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).

Article Snippet: After blocking membranes with 10 mM Tris-HCl buffer, pH 8.0, containing 150 mM sodium chloride, 0.1% Tween 20, and 5% (w/v) nonfat dry milk, IL-10 protein was detected by incubation with goat anti-human IL10 antibodies (R&D Systems, Minneapolis, MN) followed by peroxidase-conjugated donkey anti-goat secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA) and detection of bound antibody by chemiluminescence (ImmunStar WesternC kit; Bio-Rad Laboratories, Hercules, CA).

Techniques: In Vitro, Expressing, Plasmid Preparation, Cloning, Virus, Transduction, Western Blot, Marker